1. Bölüm

Grey DeLisle

Sally Avril / Betty Brant (voice)

Phil LaMarr

Rand Robertson (voice)

Robert Englund

Adrian Toomes / Vulture (voice)

Peter MacNicol

Otto Octavius (voice)

Deborah Strang

May Parker (voice)

Dee Bradley Baker

Curt Connors (voice)

John DiMaggio

Flint Marko / Hammerhead (voice)

Ben Diskin

Eddie Brock (voice)

Clancy Brown

Alex O'Hirn / Ox (voice)

Joshua LeBar

Flash Thompson (voice)

Daran Norris

J. Jonah Jameson (voice)

James Arnold Taylor

Harry Osborn (voice)

Alan Rachins

Norman Osborn (voice)

Lacey Chabert

Gwen Stacy (voice)